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czrA [2018-06-19 17:50:01]
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czrA [2018-06-19 17:50:01]

transcriptional repressor (ArsR family), cadA and czcD
locus
BSU19120
pI
5.72
mw
12.24 kDa
protein length
107 aa Sequence Blast
gene length
321 bp Sequence Blast
function
regulation of resistance against toxic metal cations
product
transcriptional repressor (ArsR family)
essential
no
synonyms
yozA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.3|Zinc]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.11|Resistance against toxic metals]

    • Gene

    Coordinates
    2,084,786 → 2,085,109

    The protein

    Protein family

  • [SW|ArsR family]

    • Effectors of protein activity

  • DNA-binding activity of CzrA is inactivated by high concentrations of toxic metal ions (induction)

    • Structure

  • [PDB|1R1U] (the apo-repressor from ''Staph. aureus'', 49% identity, 82% similarity) [Pubmed|14568530]
  • [PDB|1R1V] (the Zn(II) form from ''Staph. aureus'', 49% identity, 82% similarity) [Pubmed|14568530]
  • [PDB|2KJB] (the DNA-bound form from ''Staph. aureus'', 49% identity, 82% similarity) [Pubmed|19822742]

    • Expression and Regulation

    Operons

    genes
    [gene|6FB1865734C4DE7BE19D3D1A7E0C687B6D7094E1|czrA]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B412 (yozA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1411 NBRP B. subtilis, Japan]
  • BKE19120 (Δ[gene|6FB1865734C4DE7BE19D3D1A7E0C687B6D7094E1|czrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTATCACCTTTCTTT, downstream forward: _UP4_TGAAAAAGACATCCTTGCTT
  • BKK19120 (Δ[gene|6FB1865734C4DE7BE19D3D1A7E0C687B6D7094E1|czrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTATCACCTTTCTTT, downstream forward: _UP4_TGAAAAAGACATCCTTGCTT

    • References

  • 15948947,16430705,14568530,19822742,25213752,22007899